Rabbit GPR12 Polyclonal Antibody | anti-GPR12 antibody

GPR12 Polyclonal Antibody

Cat. Num. AAA3005884

• Gene Names: GPR12; GPCR12; GPCR21; PPP1R84
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GPR12; Polyclonal Antibody; GPR12 Polyclonal Antibody; G-protein coupled receptor 12; anti-GPR12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GPR12 polyclonal antibody detects endogenous levels of GPR12 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 334

• Applicable Applications for anti-GPR12 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 51-96 of Human GPR12.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GPR12 polyclonal antibody at 1:500 dilutionLane1:HepG2 cell lysateLane2:Raw264. 7 cell lysateLane3:Rat heart tissue lysate)

Related Product Information for anti-GPR12 antibody

G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR12 is a 334 amino acid peptide that is expressed primarily in brain, particularly in regions where neuronal differentiation takes place. GPR12 is coupled to an inhibitory G protein. It positively influences differentiation and maturation of post-mitotic neurons, and it may promote the growth of neuronal precursor cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4885295
• NCBI GeneID: 2835
• NCBI Accession #: NP_005279.1
• NCBI GenBank Nucleotide #: NP_005279.1
• UniProt Accession #: P47775
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: G-protein coupled receptor 12
• NCBI Official Synonym Full Names: G protein-coupled receptor 12
• NCBI Official Symbol: GPR12
• NCBI Official Synonym Symbols: GPCR12; GPCR21; PPP1R84
• NCBI Protein Information: G-protein coupled receptor 12
• UniProt Protein Name: G-protein coupled receptor 12
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: GPR12
• UniProt Entry Name: GPR12_HUMAN

Uniprot Description

GPR12: Promotes neurite outgrowth and blocks myelin inhibition in neurons. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; phosphatidylcholine binding

Biological Process: cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway