Soluble Fibrin Active Protein | FGA active protein

Soluble Mouse Fibrin

Cat. Num. AAA136140

• Gene Names: Fga; Fib
• Purity: >95% by SDS-PAGE analysis
• Synonyms: Soluble Fibrin; Soluble Mouse Fibrin; FGA active protein

• Host: Mouse plasma
• Purity/Purification: >95% by SDS-PAGE analysis
• Form/Format: Frozen liquid; 0.02M Acetic Acid; pH 3.6
• Concentration: 5.1 mg/ml (varies by lot)
• Sequence Length: 789

• Extintion Coefficient: 1.51
• Specific Activity: 96% Clottable
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Ships on Dry Ice.; Store at -70°C.

Related Product Information for FGA active protein

Normal mouse plasma clots are dissociated using non-denaturing conditions in which non fibrin bound plasma proteins are removed. The soluble fibrin clot is then stored under acidic conditions. The soluble fibrin will reassemble into an insoluble clot when pH is restored to neutral (e.g. by adding one-tenth volume of 10X PBS to the soluble fibrin). SDS-PAGE shows partially crosslinked fibrin with no observable free alpha, beta or gamma chain subunits. No detectable thrombin or plasmin activity is observed in the final product.

Product Categories/Family for FGA active protein

Proteins; Fibrinogen; Mouse Fibrinogen

NCBI and Uniprot Product Information

• NCBI GI #: 167555029
• NCBI GeneID: 14161
• NCBI Accession #: NP_001104518.1
• NCBI GenBank Nucleotide #: NP_001104518.1
• Molecular Weight: 340,000
• NCBI Official Full Name: fibrinogen alpha chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: Fga
• NCBI Official Synonym Symbols: Fib
• NCBI Protein Information: fibrinogen alpha chain
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: Fga

NCBI Description

This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015]

Uniprot Description

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots (PubMed:7649481). In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization (PubMed:11389004). Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood (PubMed:7649481). However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo (PubMed:10930441). Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway (PubMed:19332769). Maternal fibrinogen is essential for successful pregnancy (PubMed:7649481). Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage (PubMed:12629066). May also facilitate the immune response via both innate and T-cell mediated pathways (PubMed:23487423).