Flow Cytometry
(FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (MBS674380) Goat Anti-Mouse IgA-UNLB (MBS674422)and Goat Anti-Mouse IgM, Human ads-UNLB (MBS674352).Serially diluted Mouse IgG-APC (MBS679132) was captured and fluorescence intensity quantified.)
Mouse IgG Isotype Control
Mouse IgG-LE/AF
Synonyms
Mouse IgG Isotype Control; Mouse IgG-LE/AF; Mouse IgG - Low Endotoxin/Azide-Free; Mouse IgG Isotype Control isotype control
Host
Mouse
Isotype
Mouse IgG
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Application Notes
Quality tested applications include:
FC 7-10
ELISA 1-6
FLISA
Other referenced applications include:
IHC-PS 11
ICC 12
WB 13,14
IP 15,16
ChIP 17,18
Block 19,20
In vitro control 22-25
In vivo control 2,9, 21
Flow Cytometry FITC and BIOT conjugates <=1 ug/10 6cells
PE, PE/TXRD, APC, SPRD, PE/CY5.5, PE/CY7, APC/CY5.5, and APC/CY7 conjugates <=0.1 ug/10 6cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
ELISA : AP conjugate 1:500 – 1:5,000
Other Applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need
FC 7-10
ELISA 1-6
FLISA
Other referenced applications include:
IHC-PS 11
ICC 12
WB 13,14
IP 15,16
ChIP 17,18
Block 19,20
In vitro control 22-25
In vivo control 2,9, 21
Flow Cytometry FITC and BIOT conjugates <=1 ug/10 6cells
PE, PE/TXRD, APC, SPRD, PE/CY5.5, PE/CY7, APC/CY5.5, and APC/CY7 conjugates <=0.1 ug/10 6cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
ELISA : AP conjugate 1:500 – 1:5,000
Other Applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need
Source
Normal mouse serum
Characterization
To insure lot-to-lot consistency, each batch of product is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
Preparation and Storage
Aliquot and store at or below -20° C.
Shipping: Ships on dry ice
Shipping: Ships on dry ice
Flow Cytometry
(FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (MBS674380) Goat Anti-Mouse IgA-UNLB (MBS674422)and Goat Anti-Mouse IgM, Human ads-UNLB (MBS674352).Serially diluted Mouse IgG-APC (MBS679132) was captured and fluorescence intensity quantified.)
Flow Cytometry
(FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (MBS674380) Goat Anti-Mouse IgA-UNLB (MBS674422)and Goat Anti-Mouse IgM, Human ads-UNLB (MBS674352).Serially diluted Mouse IgG-APC (MBS679132) was captured and fluorescence intensity quantified.)
Product Categories/Family for Mouse IgG Isotype Control isotype control
Similar Products
Product Notes
The Mouse IgG Isotype Control (Catalog #AAA679134) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. Quality tested applications include: FC 7-10 ELISA 1-6 FLISA Other referenced applications include: IHC-PS 11 ICC 12 WB 13,14 IP 15,16 ChIP 17,18 Block 19,20 In vitro control 22-25 In vivo control 2,9, 21 Flow Cytometry FITC and BIOT conjugates <=1 ug/10 6cells PE, PE/TXRD, APC, SPRD, PE/CY5.5, PE/CY7, APC/CY5.5, and APC/CY7 conjugates <=0.1 ug/10 6cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL ELISA : AP conjugate 1:500 – 1:5,000 Other Applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need . Researchers should empirically determine the suitability of the Mouse IgG Isotype Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse IgG Isotype Control, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
