Rabbit anti-Human, Mouse Cdc14 Polyclonal Antibody | anti-Cdc14 antibody

Cdc14 (CDC14A, Dual specificity protein phosphatase CDC14A, CDC14 cell division cycle 14 homolog A) (PE)

Cat. Num. AAA6283675

• Gene Names: CDC14A; cdc14; DFNB32; DFNB35; hCDC14; DFNB105
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Cdc14; Polyclonal Antibody; Cdc14 (CDC14A; Dual specificity protein phosphatase CDC14A; CDC14 cell division cycle 14 homolog A) (PE); CDC14A; CDC14 cell division cycle 14 homolog A; anti-Cdc14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Cdc14 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding a portion within aa 1~379 of human cdc14.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(His6-cdc14 Antibody western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).)

Immunohistochemistry (IHC)

(Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Cdc14 antibody

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.

Product Categories/Family for anti-Cdc14 antibody

Antibodies; Cell Division Cycle (CDC)

References

Kaiser, B.K., et al. Mol. Biol. Cell 13 (7), 2289-2300 (2002). Mailand, N., et al. Nat. Cell Biol. 4 (4), 317-322 (2002). Bembenek, J. and Yu, H. J. Biol. Chem. 276 (51), 48237-48242 (2001). Li, L., et al. J. Biol. Chem. 275 (4), 2410-2414 (2000). Wong, A.K., et al. Genomics 59 (2), 248-251 (1999). Li, L., et al. J. Biol. Chem. 272 (47), 29403-29406 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 15451929
• NCBI GeneID: 8556
• NCBI Accession #: NP_003663
• NCBI GenBank Nucleotide #: NM_003672
• UniProt Accession #: Q9UNH5
• Molecular Weight: 66kDa
• NCBI Official Full Name: dual specificity protein phosphatase CDC14A isoform 1
• NCBI Official Synonym Full Names: cell division cycle 14A
• NCBI Official Symbol: CDC14A
• NCBI Official Synonym Symbols: cdc14; DFNB32; DFNB35; hCDC14; DFNB105
• NCBI Protein Information: dual specificity protein phosphatase CDC14A
• UniProt Protein Name: Dual specificity protein phosphatase CDC14A
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC14A
• UniProt Entry Name: CC14A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC14A: Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC- FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis. Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: nucleoplasm; centrosome; cytoplasm; spindle; nucleus

Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: cell proliferation; cell division; cell cycle