Typical Testing Data/Standard Curve (for reference only)
Human A kinase anchor protein 4 (AKAP4) ELISA Kit | AKAP4 elisa kit
Human A kinase anchor protein 4 (AKAP4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AKAP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKAP4 antibody and an AKAP4-HRP conjugate. The assay sample and buffer are incubated together with AKAP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP4 concentration since AKAP4 from samples and AKAP4-HRP conjugate compete for the anti-AKAP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP4 from the sample, fewer sites are left to bind AKAP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.2
Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex
Molecular Function: protein kinase A binding
Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; signal transduction; cell motility
Research Articles on AKAP4
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Product Notes
The Human AKAP4 akap4 (Catalog #AAA7202390) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7202390 ELISA Kit recognizes Human AKAP4. It is sometimes possible for the material contained within the vial of "A kinase anchor protein 4 (AKAP4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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