Mouse HK1 Antibody | anti-HK1 antibody

HK1 Antibody

Cat. Num. AAA850834

• Gene Names: HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Synonyms: HK1; HK1 Antibody; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; anti-HK1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Isotype: IgG1
• Clone Number: 7A7
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 917

• Applicable Applications for anti-HK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human HK1 expressed in E. Coli.
• Preparation and Storage: Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot

(Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.)

Immunohistochemistry

(Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) using HK1 mouse mAb with DAB staining.)

Immunofluorescence

(Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Related Product Information for anti-HK1 antibody

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

Product Categories/Family for anti-HK1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179.2
• NCBI GenBank Nucleotide #: NM_000188.2
• UniProt Accession #: P19367; O43443; O43444; O75574; Q5VTC3; Q96HC8; Q9NNZ4; Q9NNZ5; E9PCK0
• Molecular Weight: 102kDa
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
• NCBI Protein Information: hexokinase-1
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

Uniprot Description

MiscellaneousIn vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).