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Western Blot (WB) (Figure 1: Western blot analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.)

Mouse CDH2 Antibody | anti-CDH2 antibody

CDH2 Antibody

Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
CDH2; CDH2 Antibody; CDHN; NCAD; CD325; CDw325; anti-CDH2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1
Clone Number
5D5
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
906
Applicable Applications for anti-CDH2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human CDH2 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot (WB)

(Figure 1: Western blot analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.)

Western Blot (WB) (Figure 1: Western blot analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CDH2 mouse mAb)

Immunohistochemistry (IHC) (Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CDH2 mouse mAb)
Related Product Information for anti-CDH2 antibody
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
99.8kDa
NCBI Official Full Name
cadherin-2 isoform 1 preproprotein
NCBI Official Synonym Full Names
cadherin 2
NCBI Official Symbol
CDH2
NCBI Official Synonym Symbols
CDHN; NCAD; CD325; CDw325
NCBI Protein Information
cadherin-2
UniProt Protein Name
Cadherin-2
Protein Family
UniProt Gene Name
CDH2
UniProt Synonym Gene Names
CDHN; NCAD; N-cadherin

NCBI Description

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density ().

Research Articles on CDH2

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Product Notes

The CDH2 cdh2 (Catalog #AAA850756) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CDH2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IF: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the CDH2 cdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDH2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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