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Western Blot (WB) (Figure 1. Western blot analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.)

Mouse anti-Human, Mouse ApoO Antibody | anti-ApoO antibody

ApoO Antibody

Gene Names
APOO; MIC26; Mic23; My025; FAM121B
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
ApoO; ApoO Antibody; MYO25; FAM121B; MGC4825; anti-ApoO antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1
Clone Number
2F1
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
198
Applicable Applications for anti-ApoO antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of ApoO expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.)

Western Blot (WB) (Figure 1. Western blot analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human Testis tissues using ApoO mouse mAb)

Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human Testis tissues using ApoO mouse mAb)
Related Product Information for anti-ApoO antibody
ApoO. apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22.2kDa
NCBI Official Full Name
MICOS complex subunit MIC26
NCBI Official Synonym Full Names
apolipoprotein O
NCBI Official Symbol
APOO
NCBI Official Synonym Symbols
MIC26; Mic23; My025; FAM121B
NCBI Protein Information
MICOS complex subunit MIC26
UniProt Protein Name
MICOS complex subunit MIC26
Protein Family
UniProt Gene Name
APOO

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).

Research Articles on ApoO

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Product Notes

The ApoO apoo (Catalog #AAA850630) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ApoO Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ApoO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ApoO apoo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ApoO, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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