Rabbit anti-Human APOO Polyclonal Antibody | anti-APOO antibody

APOO, NT (APOO, FAM121B, Apolipoprotein O, Protein FAM121B) (PE)

Cat. Num. AAA6274543

• Gene Names: APOO; My025; FAM121B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APOO; Polyclonal Antibody; NT (APOO; FAM121B; Apolipoprotein O; Protein FAM121B) (PE); Protein FAM121B; anti-APOO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APOO
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APOO antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 9-38 in the N-terminal region of human APOO, UniProt Accession #Q9BUR5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS647348 in K562 cell line lysates (35ug/lane). This demonstrates the APOO antibody detected the APOO protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS647348 in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-APOO antibody

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.

Product Categories/Family for anti-APOO antibody

Antibodies; Lipoproteins

References

1. Bailey, S.D., et al. Diabetes Care (2010). 2. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 3. Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). 4. Lamant, M., et al. J. Biol. Chem. 281(47):36289-36302(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 13129148
• NCBI GeneID: 79135
• NCBI Accession #: NP_077027.1
• NCBI GenBank Nucleotide #: NM_024122.4
• UniProt Accession #: Q9BUR5; Q9H3J9; B2R4K9
• Molecular Weight: 20,199 Da
• NCBI Official Full Name: apolipoprotein O
• NCBI Official Synonym Full Names: apolipoprotein O
• NCBI Official Symbol: APOO
• NCBI Official Synonym Symbols: My025; FAM121B
• NCBI Protein Information: apolipoprotein O; brain my025; family with sequence similarity 121B
• UniProt Protein Name: Apolipoprotein O
• Protein Family: MICOS complex
• UniProt Gene Name: APOO
• UniProt Synonym Gene Names: FAM121B
• UniProt Entry Name: APOO_HUMAN

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

APOO: Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation. Belongs to the apolipoprotein O family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; mitochondrion; integral to membrane

Biological Process: lipid transport