Mouse anti-Human, Mouse ApoO Monoclonal Antibody | anti-ApoO antibody

ApoO Antibody

Cat. Num. AAA9602806

• Gene Names: APOO; MIC26; Mic23; My025; FAM121B
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: ApoO; Monoclonal Antibody; ApoO Antibody; 0610008C08Rik; 1110019O03Rik; Apolipoprotein O; APOO; APOO_HUMAN; FAM121B; Family with sequence similarity 121B; MGC130105; MGC130106; MGC4825; My025; MYO25; OTTHUMP00000023073; Protein FAM121B; RP23-272D10.2; anti-ApoO antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: ApoO antibody detects endogenous levels of total ApoO
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-ApoO antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000
• Immunogen: Purified recombinant fragment of human ApoO expressed in E. Coli
• Subcellular Location: Membrane. Secreted.
• Tissue Specificity: Expressed in all tissues examined. Up-regulated in diabetic heart.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.)

Related Product Information for anti-ApoO antibody

Description: ApoO: apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.
Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).
Subunit Structure: Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. he MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:25764979, PubMed:25781180, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex Interacts with IMMT/MIC60 (PubMed:25764979, PubMed:25781180). Interacts with MINOS1/MIC10 and APOOL/MIC27 (PubMed:25764979).
Post-translational Modifications: O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
Similarity: Belongs to the apolipoprotein O/MICOS complex subunit Mic27 family.

NCBI and Uniprot Product Information

• NCBI GI #: 13129148
• NCBI GeneID: 79135
• NCBI Accession #: NP_077027.1
• NCBI GenBank Nucleotide #: NM_024122.4
• UniProt Accession #: Q9BUR5; Q9H3J9; B2R4K9
• Molecular Weight: Observed: 22.2 kDa; Predicted: 23 kDa
• NCBI Official Full Name: MICOS complex subunit MIC26
• NCBI Official Synonym Full Names: apolipoprotein O
• NCBI Official Symbol: APOO
• NCBI Official Synonym Symbols: MIC26; Mic23; My025; FAM121B
• NCBI Protein Information: MICOS complex subunit MIC26
• UniProt Protein Name: MICOS complex subunit MIC26
• Protein Family: MICOS complex
• UniProt Gene Name: APOO
• UniProt Synonym Gene Names: FAM121B; MIC231 PublicationManual assertion based on opinion iniRef.11

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).