Mouse CDH1 Antibody | anti-CDH1 antibody

CDH1 Antibody

Cat. Num. AAA850097

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Reactivity: Human; Mouse; Monkey
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Synonyms: CDH1; CDH1 Antibody; UVO; CDHE; ECAD; LCAM; Arc-1; CD324; anti-CDH1 antibody

• Host: Mouse
• Reactivity: Human; Mouse; Monkey
• Isotype: IgG1
• Clone Number: 7H12
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 882

• Applicable Applications for anti-CDH1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000; ; Not yet tested in other applications.
• Immunogen: Purified recombinant fragment of human CDH1 expressed in E. Coli.
• Species: Mouse IgG1
• Preparation and Storage: Store at 4°C, for long term storage, store at -20°C.

Western Blot (WB)

(Figure 1: Western blot analysis using CDH1 mouse mAb against LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2: Immunohistochemical analysis of paraffin-embedded gastric cancer tissues (left) and lung cancer tissues (right) using CDH1 mouse mAb with DAB staining.)

Flow Cytometry (FC/FACS)

(Figure 3: Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (purple).)

Related Product Information for anti-CDH1 antibody

E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.

Product Categories/Family for anti-CDH1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 4757960
• NCBI GeneID: 999
• NCBI Accession #: NP_004351.1
• NCBI GenBank Nucleotide #: NM_004360.4
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 135kDa
• NCBI Official Full Name: cadherin-1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.