Rabbit anti-Human POU6F1 Polyclonal Antibody | anti-POU6F1 antibody

POU6F1, NT (POU6F1, BRN5, MPOU, TCFB1, POU domain, class 6, transcription factor 1, Brain-specific homeobox/POU domain protein 5, mPOU homeobox protein) (PE)

Cat. Num. AAA6336059

• Gene Names: POU6F1; BRN5; MPOU; TCFB1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POU6F1; Polyclonal Antibody; NT (POU6F1; BRN5; MPOU; TCFB1; POU domain; class 6; transcription factor 1; Brain-specific homeobox/POU domain protein 5; mPOU homeobox protein) (PE); mPOU homeobox protein; anti-POU6F1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-POU6F1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POU6F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-85 amino acids from the N-terminal region of human POU6F1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POU6F1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POU6F1 antibody detected the POU6F1 protein (arrow).)

Related Product Information for anti-POU6F1 antibody

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') (By similarity).

Product Categories/Family for anti-POU6F1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Suzuki, N., et al. Int. J. Gynecol. Cancer 20(2):212-219(2010). Yoshioka, N., et al. Hum. Cell 22(4):94-100(2009). Pereira, J.H., et al. J. Struct. Biol. 167(2):159-165(2009). Pereira, J.H., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (PT 3), 175-178 (2008): Abdel-Rahman, B., et al. Hum. Reprod. 10(10):2787-2792(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 223890225
• NCBI GeneID: 5463
• NCBI Accession #: NP_002693.3
• NCBI GenBank Nucleotide #: NM_002702.3
• UniProt Accession #: Q14863; Q15944; Q6DK47; Q7Z7P6
• Molecular Weight: 32,645 Da
• NCBI Official Full Name: POU domain, class 6, transcription factor 1
• NCBI Official Synonym Full Names: POU class 6 homeobox 1
• NCBI Official Symbol: POU6F1
• NCBI Official Synonym Symbols: BRN5; MPOU; TCFB1
• NCBI Protein Information: POU domain, class 6, transcription factor 1; brain-5; brain-specific homeobox/POU domain protein 5; brn-5; mPOU homeobox protein
• UniProt Protein Name: POU domain, class 6, transcription factor 1
• Protein Family: POU domain, class 6, transcription factor
• UniProt Gene Name: POU6F1
• UniProt Synonym Gene Names: BRN5; MPOU; TCFB1; Brain-5; Brn-5
• UniProt Entry Name: PO6F1_HUMAN

Uniprot Description

POU6F1: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). Belongs to the POU transcription factor family. Class- 6 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; nucleus; actin cytoskeleton

Molecular Function: DNA binding; transcription factor activity

Biological Process: muscle development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; heart development; brain development