Western Blot (WB)
(Western blotting of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate.)
Mouse anti-Human SIT Antibody | anti-SIT antibody
Anti-SIT Purified
Gene Names
SIT1; SIT; SIT-R
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
SIT; Anti-SIT Purified; signaling threshold regulating transmembrane adapt; SIT1; anti-SIT antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
SIT-01
Specificity
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
196
Applicable Applications for anti-SIT antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Preparation and Storage
Store at 2-8 degree C.
Western Blot (WB)
(Western blotting of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate.)
Western Blot (WB)
(Western blotting of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate.)
Related Product Information for anti-SIT antibody
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
signaling threshold-regulating transmembrane adapter 1
NCBI Official Synonym Full Names
signaling threshold regulating transmembrane adaptor 1
NCBI Official Symbol
SIT1
NCBI Official Synonym Symbols
SIT; SIT-R
NCBI Protein Information
signaling threshold-regulating transmembrane adapter 1
UniProt Protein Name
Signaling threshold-regulating transmembrane adapter 1
Protein Family
UniProt Gene Name
SIT1
UniProt Synonym Gene Names
SIT
UniProt Entry Name
SIT1_HUMAN
Uniprot Description
SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 9p13-p12
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; SH2 domain binding; kinase binding
Biological Process: immune system process; signal transduction; regulation of T cell activation
Research Articles on SIT
Similar Products
Product Notes
The SIT sit1 (Catalog #AAA1801529) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIT Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIT can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the SIT sit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIT, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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