Western Blot (WB)
(SIT1 Antibody (C-term) (MBS9203902) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SIT1 antibody detected the SIT1 protein (arrow).)
Rabbit SIT1 Polyclonal Antibody | anti-SIT1 antibody
SIT1 Antibody (C-term)
Gene Names
SIT1; SIT; SIT-R
Reactivity
HumanPredicted Reactivity: Rat
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SIT1; Polyclonal Antibody; SIT1 Antibody (C-term); Signaling threshold-regulating transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40; SIT; anti-SIT1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Rat
Predicted Reactivity: Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-189 amino acids from the C-terminal region of human SIT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
161-189
Sequence Length
196
Applicable Applications for anti-SIT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
Cellular Location
Cell membrane; Single-pass type Imembrane protein
Tissue Location
Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level) Expressed inT- and B-cell lymphoma.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(SIT1 Antibody (C-term) (MBS9203902) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SIT1 antibody detected the SIT1 protein (arrow).)
Western Blot (WB)
(SIT1 Antibody (C-term) (MBS9203902) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SIT1 antibody detected the SIT1 protein (arrow).)
Related Product Information for anti-SIT1 antibody
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
Product Categories/Family for anti-SIT1 antibody
References
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Tedoldi, S., et al. Blood 107(1):213-221(2006)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Tedoldi, S., et al. Blood 107(1):213-221(2006)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21126
NCBI Official Full Name
signaling threshold-regulating transmembrane adapter 1
NCBI Official Synonym Full Names
signaling threshold regulating transmembrane adaptor 1
NCBI Official Symbol
SIT1
NCBI Official Synonym Symbols
SIT; SIT-R
NCBI Protein Information
signaling threshold-regulating transmembrane adapter 1
UniProt Protein Name
Signaling threshold-regulating transmembrane adapter 1
Protein Family
UniProt Gene Name
SIT1
UniProt Synonym Gene Names
SIT
UniProt Entry Name
SIT1_HUMAN
Uniprot Description
SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 9p13-p12
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; SH2 domain binding; kinase binding
Biological Process: immune system process; signal transduction; regulation of T cell activation
Research Articles on SIT1
Similar Products
Product Notes
The SIT1 sit1 (Catalog #AAA9203902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 161-189. The SIT1 Antibody (C-term) reacts with Human Predicted Reactivity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the SIT1 sit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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