Mouse anti-Human MDM4 Antibody | anti-MDM4 antibody

MDM4 Antibody

Cat. Num. AAA850875

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Synonyms: MDM4; MDM4 Antibody; HDMX; MDMX; MRP1; anti-MDM4 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: 2D10F4
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 440

• Applicable Applications for anti-MDM4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human MDM4 expressed in E. Coli.
• Preparation and Storage: Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.)

Immunohistochemistry (IHC)

(Figure 3. Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using MDM4 mouse mAb)

Related Product Information for anti-MDM4 antibody

MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.

Product Categories/Family for anti-MDM4 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 323510634
• NCBI GeneID: 4194
• NCBI Accession #: NP_001191100.1
• NCBI GenBank Nucleotide #: NM_001204171.1
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 55kDa
• NCBI Official Full Name: protein Mdm4 isoform 2
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4
• UniProt Protein Name: Protein Mdm4
• Protein Family: Protein
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX

NCBI Description

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.