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Western Blot (WB) (Figure 1. Western blot analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).)

Mouse anti-Human ELK1 Antibody | anti-ELK1 antibody

ELK1 Antibody

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
ELK1; ELK1 Antibody; anti-ELK1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
3H6D12; 4H9C8; 4H9F1
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
428
Applicable Applications for anti-ELK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of ELK1 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).)

Western Blot (WB) (Figure 1. Western blot analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human brain tumor tissue, showing nuclear and cytoplasmic localization using ELK1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human brain tumor tissue, showing nuclear and cytoplasmic localization using ELK1 mouse mAb with DAB staining.)
Related Product Information for anti-ELK1 antibody
The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily, which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus, It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,217 Da
NCBI Official Full Name
ETS domain-containing protein Elk-1 isoform a
NCBI Official Synonym Full Names
ELK1, ETS transcription factor
NCBI Official Symbol
ELK1
NCBI Protein Information
ETS domain-containing protein Elk-1
UniProt Protein Name
ETS domain-containing protein Elk-1
UniProt Gene Name
ELK1

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation ().

Research Articles on ELK1

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Product Notes

The ELK1 elk1 (Catalog #AAA850139) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ELK1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ELK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ELK1 elk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELK1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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