Rabbit Elk1 Polyclonal Antibody | anti-Elk1 antibody

Elk1 antibody

Cat. Num. AAA5309483

• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Elk1; Polyclonal Antibody; Elk1 antibody; Rabbit polyclonal Elk1 antibody; Polyclonal Elk1 antibody; Anti-Elk1 antibody; Elk-1; Elk 1 antibody; Elk-1 antibody; Phospho Ser389 antibody; Elk 1; anti-Elk1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 428

• Applicable Applications for anti-Elk1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000
• Immunogen: Elk1 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from Human Elk-1 as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage.; Aliquot and store at-20 degree C for long term storage.; Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Phospho-Ser389) antibody (left) or the same antibody preincubated with with blocking peptide (right))

Western Blot (WB)

(Western Blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using Elk1 (Phospho-Ser389) antibody)

Related Product Information for anti-Elk1 antibody

Elk1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene.

Product Categories/Family for anti-Elk1 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4126584
• NCBI GeneID: 2002
• Molecular Weight: 11,217 Da
• NCBI Official Full Name: elk1
• NCBI Official Synonym Full Names: ELK1, ETS transcription factor
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1
• UniProt Protein Name: ETS domain-containing protein Elk-1
• Protein Family: ETS domain-containing protein
• UniProt Gene Name: ELK1

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation ().