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Western Blot (WB) (Figure 1. Western blot analysis using CHIT1 mouse mAb against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).)

Mouse anti-Human CHIT1 Antibody | anti-CHIT1 antibody

CHIT1 Antibody

Gene Names
CHIT1; CHI3; CHIT; CHITD
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
CHIT1; CHIT1 Antibody; Chitotriosidase; CHI3; CHIT; FLJ00314; MGC125322; anti-CHIT1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
1D9G2; 1D9G4; 2B4G3
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
447
Applicable Applications for anti-CHIT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of CHIT1 (aa22-137) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using CHIT1 mouse mAb against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).)

Western Blot (WB) (Figure 1. Western blot analysis using CHIT1 mouse mAb against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).)
Related Product Information for anti-CHIT1 antibody
CHIT1.chitinase 1 (chitotriosidase). Chitotriosidase is a 50 kDa secreted glycoprotein that belongs to glycohydrolase family # 18. It is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,613 Da
NCBI Official Full Name
chitotriosidase-1 isoform 2
NCBI Official Synonym Full Names
chitinase 1
NCBI Official Symbol
CHIT1
NCBI Official Synonym Symbols
CHI3; CHIT; CHITD
NCBI Protein Information
chitotriosidase-1
UniProt Protein Name
Chitotriosidase-1
Protein Family
UniProt Gene Name
CHIT1

NCBI Description

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.

Research Articles on CHIT1

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Product Notes

The CHIT1 chit1 (Catalog #AAA850169) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CHIT1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHIT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the CHIT1 chit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHIT1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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