Mouse anti-Human CDX2/Caudal Type Homeobox 2 Antibody | anti-CDX2 antibody

CDX2/Caudal Type Homeobox 2 (GI Epithelial Marker)

Cat. Num. AAA4380782

• Gene Names: CDX2; CDX3; CDX-3; CDX2/AS
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CDX2/Caudal Type Homeobox 2; CDX2/Caudal Type Homeobox 2 (GI Epithelial Marker); Caudal type homeobox 2; Caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX2; anti-CDX2 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rCDX2/1690
• Specificity: The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 313

• Applicable Applications for anti-CDX2 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human colon carcinoma (IHC).
• Immunogen: Recombinant fragment (around aa150-249) of human CDX2 protein (exact sequence is proprietary)
• Cellular Localization: Nuclear
• Hu-chromosome Location: 13q12.2
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with CDX2 Mouse Monoclonal Antibody (rCDX2/1690).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with CDX2 Mouse Monoclonal Antibody (rCDX2/1690).)

SDS-Page

(SDS-PAGE Analysis Purified CDX2 Mouse Monoclonal Antibody (rCDX2/1690). Confirmation of Purity and Integrity of Antibody.)

References

1. Mallo, G.V., et al. 1998. Expression of the Cdx1 and Cdx2 homeotic genes leads to reduced malignancy in colon cancer-derived cells. J. Biol. Chem. 273: 14030-14036. 2. Silberg, D.G., et al. 2000. Cdx1 and cdx2 expression during intestinal development. Gastroenterology 119: 961-971

NCBI and Uniprot Product Information

• NCBI GI #: 372624392
• NCBI GeneID: 1045
• NCBI Accession #: NP_001256.3
• NCBI GenBank Nucleotide #: NP_001256.3
• UniProt Accession #: Q99626
• Molecular Weight: 40kDa
• NCBI Official Full Name: homeobox protein CDX-2 isoform 1
• NCBI Official Synonym Full Names: caudal type homeobox 2
• NCBI Official Symbol: CDX2
• NCBI Official Synonym Symbols: CDX3; CDX-3; CDX2/AS
• NCBI Protein Information: homeobox protein CDX-2
• UniProt Protein Name: Homeobox protein CDX-2
• Protein Family: Homeobox protein
• UniProt Gene Name: CDX2
• UniProt Synonym Gene Names: CDX3
• UniProt Entry Name: CDX2_HUMAN

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]

Uniprot Description

CDX2: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Belongs to the Caudal homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; condensed nuclear chromosome; transcriptional repressor complex

Molecular Function: double-stranded DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of epithelial cell polarity; anterior/posterior pattern formation; blood vessel development; organ morphogenesis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; endosome to lysosome transport; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; trophectodermal cell differentiation