Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Jukat cell lysate Lane 4. : MCF7 cell lysate Lane 5. : A549 cell lysate)
Mouse anti-Human PMVK Monoclonal Antibody | anti-PMVK antibody
PMVK antibody
Gene Names
PMVK; PMK; PMKA; PMKASE; POROK1; HUMPMKI
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
PMVK; Monoclonal Antibody; PMVK antibody; Phosphomevalonate kinase; HuMPMKI; PMK; PMKA; PMKASE; anti-PMVK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT21C10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
192
Applicable Applications for anti-PMVK antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Antigen Species
Human
Immunogen
Recombinant human PMVK (1-192aa) purified from E. coli
Clone Note
Anti-human PMVK mAb, clone AT21C10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PMVK protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Jukat cell lysate Lane 4. : MCF7 cell lysate Lane 5. : A549 cell lysate)
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Jukat cell lysate Lane 4. : MCF7 cell lysate Lane 5. : A549 cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-PMVK antibody
Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
Product Categories/Family for anti-PMVK antibody
References
Cho YK., et al. (2001) J Biol chem. 278: 4510-4515.; Hogenboom S., et al. (2004) J Lipid Res. 45: 697-705.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,995 Da
NCBI Official Full Name
phosphomevalonate kinase isoform 1
NCBI Official Synonym Full Names
phosphomevalonate kinase
NCBI Official Symbol
PMVK
NCBI Official Synonym Symbols
PMK; PMKA; PMKASE; POROK1; HUMPMKI
NCBI Protein Information
phosphomevalonate kinase
UniProt Protein Name
Phosphomevalonate kinase
UniProt Gene Name
PMVK
UniProt Synonym Gene Names
PMKI; PMKase; hPMK
NCBI Description
This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
Research Articles on PMVK
Similar Products
Product Notes
The PMVK pmvk (Catalog #AAA200352) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PMVK antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PMVK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the PMVK pmvk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMVK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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