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Western Blot (WB) (Figure 1: Western blot analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.)

Mouse anti-Human CD69 Antibody | anti-CD69 antibody

CD69 Antibody

Gene Names
BTN1A1; BT; BTN; BTN1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
CD69; CD69 Antibody; CLEC2C; anti-CD69 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
8B6
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
526
Applicable Applications for anti-CD69 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human CD69 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot (WB)

(Figure 1: Western blot analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.)

Western Blot (WB) (Figure 1: Western blot analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-CD69 mouse mAb)

Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-CD69 mouse mAb)

Flow Cytometry (FC/FACS)

(Figure 3: Flow cytometric analysis of Jurkat cells using CD69 mouse mAb (green) and negative control (purple).)

Flow Cytometry (FC/FACS) (Figure 3: Flow cytometric analysis of Jurkat cells using CD69 mouse mAb (green) and negative control (purple).)
Related Product Information for anti-CD69 antibody
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, andplatelets Subcellular location: Membrane, Single-pass type II membrane protein Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhanscells and platelets Sequence similarities: Contains 1 C-type lectin domain.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
696
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22.5kDa
NCBI Official Full Name
butyrophilin subfamily 1 member A1
NCBI Official Synonym Full Names
butyrophilin subfamily 1 member A1
NCBI Official Symbol
BTN1A1
NCBI Official Synonym Symbols
BT; BTN; BTN1
NCBI Protein Information
butyrophilin subfamily 1 member A1
UniProt Protein Name
Butyrophilin subfamily 1 member A1
Protein Family
UniProt Gene Name
BTN1A1
UniProt Synonym Gene Names
BTN; BT

NCBI Description

Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq, Jul 2008]

Uniprot Description

May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion ().

Research Articles on CD69

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Product Notes

The CD69 btn1a1 (Catalog #AAA850412) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD69 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the CD69 btn1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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