Mouse anti-Human CD69 Monoclonal Antibody | anti-CD69 antibody

Anti-CD69 Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8119015

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD69; Monoclonal Antibody; Anti-CD69 Antibody (APC); Mouse Monoclonal; CD69 Antibody (APC); Mouse MAb; Anti-AIM Antibody; Anti-BL-AC/P26 Antibody; Anti-CLEC2C Antibody; Anti-EA1 Antibody; Anti-GP32/28 Antibody; Anti-MLR-3 Antibody; CD69 molecule; anti-CD69 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 6
• Specificity: Human CD69
• Purity/Purification: Protein A
• Form/Format: Liquid. Aqueous solution containing 0.5% BSA and 0.03% ProClin300
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD69 antibody: Flow Cytometry (FC/FACS)
• Immunogen: Recombinant Human CD69 Protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD69 (rh CD69; NP_001772.1; Ser62-Lys199) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD69 expression on PHA-activated human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human CD69. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Related Product Information for anti-CD69 antibody

Background: Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator, potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.

References

Ziegler SF, et al. (1994) The activation antigen CD69. Stem Cells. 12(5): 456-65.Marzio R, et al. (1999) CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol. 21(3): 565-82.Lamana A, et al. (2006) The role of CD69 in acute neutrophil-mediated inflammation. Eur J Immunol. 36(10): 2632-8.Miki-Hosokawa T, et al. (2009) CD69 controls the pathogenesis of allergic airway inflammation. J Immunol. 183(12): 8203-15.