Mouse anti-Human CD24 Antibody | anti-CD24 antibody

Anti-Hu CD24 Purified

Cat. Num. AAA1801543

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD24; Anti-Hu CD24 Purified; CD24 molecule; Small cell lung carcinoma cluster 4 antigen; HSA; nectadorin; anti-CD24 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: SN3
• Specificity: The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 80

• Applicable Applications for anti-CD24 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, MC (CyTOF)
• Application Notes: FC/FACS: QC tested
• Immunogen: Glycoproteins purified from human NALM-1 cell line.
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD24 (SN3) purified, GAM-APC.)

Related Product Information for anti-CD24 antibody

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

NCBI and Uniprot Product Information

• NCBI GI #: 143811372
• UniProt Accession #: P25063
• NCBI Official Full Name: Signal transducer CD24
• UniProt Protein Name: Signal transducer CD24
• Protein Family: Signal transducer
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A
• UniProt Entry Name: CD24_HUMAN

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Membrane protein, GPI anchor; Cell surface; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function: protein binding; signal transducer activity; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; cholesterol homeostasis; cell-cell adhesion; elevation of cytosolic calcium ion concentration; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; negative regulation of transforming growth factor-beta3 production; T cell costimulation; regulation of epithelial cell differentiation; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To