Flow Cytometry (FC/FACS)
(Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using mouse monoclonal anti-c-Myc (9E10) FITC.)
Mouse c-Myc Tag Antibody
Anti-c-Myc Tag FITC
Reactivity
Tagged fusion proteins in all species, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
c-Myc Tag; Anti-c-Myc Tag FITC; Cellular myelocytomatosis oncogene; bHLH; MRTL; MYCC; anti-c-Myc Tag antibody
Host
Mouse
Reactivity
Tagged fusion proteins in all species, Human
Isotype
IgG1
Clone Number
9E10
Specificity
The antibody 9E10 can be used to detect the c-Myc tag.
Purity/Purification
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
11
Applicable Applications for anti-c-Myc Tag antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Conjugation
FITC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using mouse monoclonal anti-c-Myc (9E10) FITC.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using mouse monoclonal anti-c-Myc (9E10) FITC.)
Related Product Information for anti-c-Myc Tag antibody
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
c-myc protein, partial
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Product Notes
The c-Myc Tag (Catalog #AAA1800304) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-c-Myc Tag FITC reacts with Tagged fusion proteins in all species, Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Myc Tag can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the c-Myc Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Myc Tag, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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