Rat KEAP1/Kelch-Like ECH-Associated Protein 1 ELISA Kit | KEAP1 elisa kit

Rat KEAP1/Kelch-Like ECH-Associated Protein 1 ELISA Kit

Cat. Num. AAA2890839

• Gene Names: Keap1; Inrf2
• Reactivity: Rat
• Synonyms: KEAP1/Kelch-Like ECH-Associated Protein 1; Rat KEAP1/Kelch-Like ECH-Associated Protein 1 ELISA Kit; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Inrf2; KEAP1 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Pig Oxidized low-density lipoprotein receptor 1
• Sequence Length: 624

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.78-50 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.9%
• Inter-assay Precision: Inter-Assay CV: <=9.8%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KEAP1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13431599
• NCBI GeneID: 117519
• UniProt Accession #: P57790
• NCBI Official Full Name: Kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: Kelch-like ECH-associated protein 1
• NCBI Official Symbol: Keap1
• NCBI Official Synonym Symbols: Inrf2
• NCBI Protein Information: kelch-like ECH-associated protein 1
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• Protein Family: Kelch-like ECH-associated protein
• UniProt Gene Name: Keap1
• UniProt Synonym Gene Names: Inrf2; INrf2

NCBI Description

binds nuclear transcription factor Nrf2 and retains it in the cytoplasm thus inhibiting it from functioning in the nucleus [RGD, Feb 2006]

Uniprot Description

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.

Protein type: Endoplasmic reticulum; Motility/polarity/chemotaxis; Transcription regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: actin filament; cell-cell adherens junction; cytoplasm; cytosol; endoplasmic reticulum; focal adhesion; microtubule organizing center; midbody; nucleoplasm; nucleus; protein complex

Molecular Function: protein binding; protein homodimerization activity; transcription factor binding; ubiquitin-protein transferase activity

Biological Process: cytoplasmic sequestering of transcription factor; in utero embryonic development; negative regulation of transcription factor activity; proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein oligomerization; protein ubiquitination; regulation of epidermal cell differentiation; regulation of transcription, DNA-templated; response to metal ion; selenium compound metabolic process; transcription, DNA-dependent