Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Wolframin (WFS1) Standard Curve.)
Mouse Wolframin (WFS1) ELISA Kit | WFS1 elisa kit
Mouse Wolframin (WFS1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Wolframin (WFS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Wolframin (WFS1) Standard Curve.)
Background: Human WHDC1 expressed in insect cells had an apparent molecular mass of about 100 kD by SDS-PAGE. Western blot analysis detected WHDC1 in most human and mouse organs examined, with highest expression in brain. WHDC1 was also expressed in all cultured cell lines tested. WHDC1 partitioned predominantly in the membrane fraction of COS-7 cells, and it appeared to be a peripheral membrane protein, since high salt or alkaline pH released it into the soluble fraction. Immunohistochemical analysis localized WHDC1 primarily to the perinuclear compartment, near the microtubule-organizing center. In some cells, it also localized to tubulovesicular structures in the cell periphery. WHDC1 colocalized extensively with the cis-Golgi marker GM130 (GOLGA2), but not with medial- or trans-Golgi markers.
NCBI and Uniprot Product Information
Uniprot Description
WFS1: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.
Protein type: Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5|5 B3
Cellular Component: endoplasmic reticulum; integral component of endoplasmic reticulum membrane; integral component of membrane; membrane; proteasome complex
Molecular Function: activating transcription factor binding; ATPase binding; calcium-dependent protein binding; calmodulin binding; ubiquitin protein ligase binding
Biological Process: calcium ion homeostasis; endoplasmic reticulum calcium ion homeostasis; endoplasmic reticulum unfolded protein response; ER-associated protein catabolic process; glucose homeostasis; kidney development; negative regulation of ATF6-mediated unfolded protein response; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of neuron apoptosis; negative regulation of programmed cell death; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of type B pancreatic cell apoptotic process; neurological system process; positive regulation of adenylate cyclase activity; positive regulation of calcium ion transport; positive regulation of growth; positive regulation of protein metabolic process; positive regulation of protein ubiquitination; protein stabilization; regulation of cell cycle; renal water homeostasis; sensory perception of sound; visual perception
Research Articles on WFS1
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Product Notes
The Mouse WFS1 wfs1 (Catalog #AAA9716624) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716624 ELISA Kit recognizes Mouse WFS1. It is sometimes possible for the material contained within the vial of "Wolframin (WFS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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