Mouse WNT4 (Protein Wnt-4) ELISA Kit | WNT4 elisa kit

Mouse WNT4 (Protein Wnt-4) ELISA Kit

Cat. Num. AAA7606730

• Gene Names: WNT4; WNT-4; SERKAL
• Reactivity: Mouse
• Synonyms: WNT4 (Protein Wnt-4); Mouse WNT4 (Protein Wnt-4) ELISA Kit; Protein Wnt-4; WNT4 elisa kit

• Reactivity: Mouse
• Sequence Length: 351

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- WNT4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-WNT4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the WNT4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of WNT4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 17402922
• NCBI GeneID: 54361
• NCBI Accession #: NP_110388.2
• NCBI GenBank Nucleotide #: NM_030761.4
• UniProt Accession #: P56705; Q5TZQ0; Q96T81; Q9BXF5; Q9H1J8; Q9UJM2; B4DJF9
• Molecular Weight: 32,954 Da
• NCBI Official Full Name: protein Wnt-4
• NCBI Official Synonym Full Names: Wnt family member 4
• NCBI Official Symbol: WNT4
• NCBI Official Synonym Symbols: WNT-4; SERKAL
• NCBI Protein Information: protein Wnt-4
• UniProt Protein Name: Protein Wnt-4
• UniProt Gene Name: WNT4

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity

Biological Process: adrenal gland development; androgen biosynthetic process; cell fate commitment; epithelial to mesenchymal transition; female gonad development; female sex determination; kidney development; liver development; male gonad development; negative regulation of transcription, DNA-dependent; neuron differentiation; positive regulation of aldosterone biosynthetic process; positive regulation of bone mineralization; positive regulation of collagen biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs; Mullerian Aplasia And Hyperandrogenism