Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vitamin D 25-hydroxylase (CYP2R1) Standard Curve.)
Human Vitamin D 25-Hydroxylase (CYP2R1) ELISA Kit | CYP2R1 elisa kit
Human Vitamin D 25-Hydroxylase (CYP2R1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vitamin D 25-hydroxylase (CYP2R1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vitamin D 25-hydroxylase (CYP2R1) Standard Curve.)
Background: CYP2R1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in CYP2R1 has been associated with selective 25-hydroxyvitamin D deficiency.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family.
Protein type: EC 1.14.13.15; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p15.2
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm
Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity
Biological Process: steroid metabolic process; organic acid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process
Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b
Research Articles on CYP2R1
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Product Notes
The Human CYP2R1 cyp2r1 (Catalog #AAA9715827) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715827 ELISA Kit recognizes Human CYP2R1. It is sometimes possible for the material contained within the vial of "Vitamin D 25-Hydroxylase (CYP2R1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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