Mouse Visfatin (VF) ELISA Kit | VF elisa kit

Mouse Visfatin (VF) ELISA Kit

Cat. Num. AAA2097765

• Gene Names: Nampt; Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik
• Reactivity: Mouse
• Synonyms: Visfatin (VF); Mouse Visfatin (VF) ELISA Kit; Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase; VF elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Visfatin (VF).; No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
• Sequence Length: 491

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.23-100ng/mL
• Sensitivity: < 0.44ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VF elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Visfatin (VF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Visfatin (VF) and unlabeled Visfatin (VF) (Standards or samples) with the pre-coated antibody specific to Visfatin (VF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Visfatin (VF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Visfatin (VF) in the sample.

Product Categories/Family for VF elisa kit

Metabolic pathway; Cardiovascular biology; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 257153454
• NCBI GeneID: 59027
• NCBI Accession #: NP_067499.2
• NCBI GenBank Nucleotide #: NM_021524.2
• UniProt Accession #: Q99KQ4; Q8C3B5; Q9JKM0
• Molecular Weight: 55,447 Da
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: Nampt
• NCBI Official Synonym Symbols: Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: Nampt
• UniProt Synonym Gene Names: Pbef1; NAmPRTase; Nampt; PBEF

Uniprot Description

The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.