Mouse Vacuolar protein sorting-associated protein 4A (VPS4A) ELISA Kit | VPS4A elisa kit

Mouse Vacuolar protein sorting-associated protein 4A (VPS4A) ELISA Kit

Cat. Num. AAA286705

• Gene Names: Vps4a; AI325971; AW553189; 4930589C15Rik
• Reactivity: Mouse
• Synonyms: Vacuolar protein sorting-associated protein 4A (VPS4A); Mouse Vacuolar protein sorting-associated protein 4A (VPS4A) ELISA Kit; FLJ22197; SKD1; SKD1A; SKD2; VPS4; VPS4-1; SKD1-homolog; vacuolar protein sorting factor 4A; vacuolar sorting protein 4; VPS4A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse VPS4A. No significant cross-reactivity or interference between Mouse VPS4A and analogues was observed.
• Sequence Length: 437

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for VPS4A elisa kit

This assay employs a two-site sandwich ELISA to quantitate VPS4A in samples. An antibody specific for VPS4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VPS4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 18699726
• NCBI GeneID: 116733
• NCBI Accession #: NP_569053.1
• NCBI GenBank Nucleotide #: NM_126165.1
• UniProt Accession #: Q8VEJ9; Q3TXT2
• Molecular Weight: 48,907 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 4A
• NCBI Official Synonym Full Names: vacuolar protein sorting 4A
• NCBI Official Symbol: Vps4a
• NCBI Official Synonym Symbols: AI325971; AW553189; 4930589C15Rik
• NCBI Protein Information: vacuolar protein sorting-associated protein 4A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps4a

Uniprot Description

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan ().