Rabbit anti-Human SHROOM2 Polyclonal Antibody | anti-SHROOM2 antibody

SHROOM2, ID (SHROOM2, APXL, Protein Shroom2, Apical-like protein, Protein APXL) (Biotin)

Cat. Num. AAA6347496

• Gene Names: SHROOM2; APXL; HSAPXL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SHROOM2; Polyclonal Antibody; ID (SHROOM2; APXL; Protein Shroom2; Apical-like protein; Protein APXL) (Biotin); Protein APXL; anti-SHROOM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SHROOM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SHROOM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-625 amino acids from the Central region of human SHROOM2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SHROOM2 Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the SHROOM2 antibody detected the SHROOM2 protein (arrow).)

Related Product Information for anti-SHROOM2 antibody

The protein encoded by this gene shares significant similarities with the apical protein from Xenopus laevis which is implicated in amiloride-sensitive sodium channel activity. This gene is a strong candidate gene for ocular albinism type 1 syndrome.

Product Categories/Family for anti-SHROOM2 antibody

Antibodies; Disease Markers

References

Wu, C., et al. Proteomics 7(11):1775-1785(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Dietz, M.L., et al. J. Biol. Chem. 281(29):20542-20554(2006). Hagens, O., et al. BMC Cell Biol. 7, 18 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4502175
• NCBI GeneID: 357
• NCBI Accession #: NP_001640.1
• NCBI GenBank Nucleotide #: NM_001649.2
• UniProt Accession #: Q13796; B9EIQ7
• Molecular Weight: 176,410 Da
• NCBI Official Full Name: protein Shroom2
• NCBI Official Synonym Full Names: shroom family member 2
• NCBI Official Symbol: SHROOM2
• NCBI Official Synonym Symbols: APXL; HSAPXL
• NCBI Protein Information: protein Shroom2; apical-like protein; APX homolog of Xenopus
• UniProt Protein Name: Protein Shroom2
• Protein Family: Protein
• UniProt Gene Name: SHROOM2
• UniProt Synonym Gene Names: APXL
• UniProt Entry Name: SHRM2_HUMAN

NCBI Description

The protein encoded by this gene shares significant similarities with the apical protein from Xenopus laevis which is implicated in amiloride-sensitive sodium channel activity. This gene is a strong candidate gene for ocular albinism type 1 syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

APXL: May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution. Belongs to the shroom family.

Protein type: Channel, sodium

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: filamentous actin; microtubule; cortical actin cytoskeleton; tight junction; cell-cell adherens junction; cytoskeleton; cell soma; apical plasma membrane; plasma membrane

Molecular Function: actin filament binding; amiloride-sensitive sodium channel activity; protein domain specific binding; protein binding; beta-catenin binding; actin binding

Biological Process: eye pigment granule organization and biogenesis; actin filament bundle formation; cell migration; camera-type eye development; cell morphogenesis; negative regulation of actin filament depolymerization; ear development; cellular pigment accumulation; apical protein localization; camera-type eye morphogenesis; melanosome organization and biogenesis; establishment of melanosome localization; lens morphogenesis in camera-type eye; brain development; intercellular junction maintenance