Mouse Ubiquitin thioesterase OTU1 (YOD1) ELISA Kit | YOD1 elisa kit
Mouse Ubiquitin thioesterase OTU1 (YOD1) ELISA Kit
Principle of the Assay: YOD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YOD1 antibody and an YOD1-HRP conjugate. The assay sample and buffer are incubated together with YOD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YOD1 concentration since YOD1 from samples and YOD1-HRP conjugate compete for the anti-YOD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YOD1 from the sample, fewer sites are left to bind YOD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YOD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Uniprot Description
YOD1: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin-specific protease; C2H2-type zinc finger protein; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 3.4.19.12; Lipid Metabolism - unsaturated fatty acid biosynthesis; Protease
Chromosomal Location of Human Ortholog: 1q32.2
Molecular Function: protein binding; metal ion binding; ubiquitin-specific protease activity
Biological Process: ER-associated protein catabolic process; unfolded protein response
Research Articles on YOD1
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Product Notes
The Mouse YOD1 yod1 (Catalog #AAA7249955) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249955 ELISA Kit recognizes Mouse YOD1. It is sometimes possible for the material contained within the vial of "Ubiquitin thioesterase OTU1 (YOD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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