Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylinositol-binding clathrin assembly protein (PICALM) Standard Curve.)
Human Phosphatidylinositol-Binding Clathrin Assembly Protein (PICALM) ELISA Kit | PICALM elisa kit
Human Phosphatidylinositol-Binding Clathrin Assembly Protein (PICALM) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylinositol-binding clathrin assembly protein (PICALM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylinositol-binding clathrin assembly protein (PICALM) Standard Curve.)
Background: Dreyling et al. (1996) identified a breakpoint on chromosome 11 involving the previously unknown gene, CALM. CALM has a very high homology to the murine clathrin assembly protein ap3. Mao et al. (2001) determined the crystal structure of the N-terminal domain (the NAP domain) of the Drosophila Lap protein, which is homologous to human PICALM. The structure revealed a novel fold consisting of a large double layer of sheets of 10 alpha helices and a unique site for binding phosphoinositides.This motif is found in other proteins predicted to have domains of similar structure (e.g., Huntingtin-interacting protein-1). The structure is in part similar to the epsin N-terminal (ENTH) domain, but epsin lacks the phosphatidylinositol-4,5-bisphosphate-binding site.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Uniprot Description
PICALM: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Vesicle
Chromosomal Location of Human Ortholog: 11q14
Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; intracellular membrane-bound organelle; membrane; cell soma; perinuclear region of cytoplasm; AP-2 adaptor complex; coated pit; nucleus; clathrin coat of coated pit; vesicle
Molecular Function: protein binding; clathrin binding; clathrin heavy chain binding; phosphatidylinositol binding
Biological Process: receptor-mediated endocytosis; synaptic vesicle maturation; dendrite morphogenesis; positive regulation of transcription, DNA-dependent; endosome transport; vesicle-mediated transport; clathrin cage assembly; cell proliferation; iron ion homeostasis; regulation of protein localization; axonogenesis; receptor internalization; regulation of endocytosis; negative regulation of receptor-mediated endocytosis; hemopoiesis; protein complex assembly
Disease: Leukemia, Acute Myeloid
Research Articles on PICALM
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Product Notes
The Human PICALM picalm (Catalog #AAA9714878) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714878 ELISA Kit recognizes Human PICALM. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-Binding Clathrin Assembly Protein (PICALM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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