Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein phosphatase non-receptor type 4 (PTPN4) Standard Curve.)
Mouse Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4) ELISA Kit | PTPN4 elisa kit
Mouse Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4) ELISA Kit
Gene Names
Ptpn4; TEP; Ptn4; PTPMEG; hPTP-MEG; TEP/mPTPMEG
Reactivity
Mouse
Synonyms
Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4); Mouse Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4) ELISA Kit; PTPN4; PTPMEG; PTPMEG1; PTPase-MEG1; megakaryocyte phosphatase; megakaryocyte protein-tyrosine phosphatase; protein tyrosine phosphatase MEG1; protein tyrosine phosphatase; non-receptor type 4; PTPN4 elisa kit
Reactivity
Mouse
Specificity
Mouse Tyrosine-protein phosphatase non-receptor type 4 (PTPN4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTPN4. No significant cross-reactivity or interference between Mouse PTPN4 and analogues was observed.
Sequence Length
926
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein phosphatase non-receptor type 4 (PTPN4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein phosphatase non-receptor type 4 (PTPN4) Standard Curve.)
Related Product Information for PTPN4 elisa kit
Description: This Mouse Tyrosine-protein phosphatase non-receptor type 4 (PTPN4) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPN4 in samples. An antibody specific for PTPN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tyrosine-protein phosphatase non-receptor type 4 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
Background: Tyrosine-protein phosphatase non-receptor type 4 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 4
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 4
NCBI Official Symbol
Ptpn4
NCBI Official Synonym Symbols
TEP; Ptn4; PTPMEG; hPTP-MEG; TEP/mPTPMEG
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 4
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 4
Protein Family
UniProt Gene Name
Ptpn4
UniProt Entry Name
PTN4_MOUSE
Uniprot Description
PTPN4: May act at junctions between the membrane and the cytoskeleton. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)
Cellular Component: internal side of plasma membrane; cytoskeleton; membrane; cytoplasm; plasma membrane
Molecular Function: protein binding; hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; phosphoric monoester hydrolase activity; cytoskeletal protein binding; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity
Biological Process: dephosphorylation; protein amino acid dephosphorylation
Research Articles on PTPN4
Similar Products
Product Notes
The Mouse PTPN4 ptpn4 (Catalog #AAA9717222) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717222 ELISA Kit recognizes Mouse PTPN4. It is sometimes possible for the material contained within the vial of "Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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