Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) Standard Curve.)
Mouse Tumor Necrosis Factor Ligand Superfamily Member 13 (TNFSF13) ELISA Kit | TNFSF13 elisa kit
Mouse Tumor Necrosis Factor Ligand Superfamily Member 13 (TNFSF13) ELISA Kit
Gene Names
Tnfsf13; April; Tall2; Trdl1; Tnlg7b; 2310026N09Rik
Reactivity
Mouse
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 13 (TNFSF13); Mouse Tumor Necrosis Factor Ligand Superfamily Member 13 (TNFSF13) ELISA Kit; TNFSF13; APRIL; CD256; TALL2; TRDL-1; UNQ383/PRO715; ligand; TNF-and APOL-related leukocyte expressed ligand 2; a proliferation inducing ligand; tumor necrosis factor (ligand) superfamily member 13 transcript; TNFSF13 elisa kit
Reactivity
Mouse
Specificity
Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF13. No significant cross-reactivity or interference between Mouse TNFSF13 and analogues was observed.
Sequence Length
240
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) Standard Curve.)
Related Product Information for TNFSF13 elisa kit
Description: This Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13 in samples. An antibody specific for TNFSF13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tumor necrosis factor ligand superfamily member 13 is a member of the tumor necrosis factor (TNF) ligand family. Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Background: Tumor necrosis factor ligand superfamily member 13 is a member of the tumor necrosis factor (TNF) ligand family. Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13 isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13
NCBI Official Symbol
Tnfsf13
NCBI Official Synonym Symbols
April; Tall2; Trdl1; Tnlg7b; 2310026N09Rik
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13
Protein Family
UniProt Gene Name
Tnfsf13
UniProt Synonym Gene Names
April; APRIL
UniProt Entry Name
TNF13_MOUSE
Uniprot Description
TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; external side of plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: regulation of immune response; immune system process; immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; immune response; immunoglobulin mediated immune response; positive regulation of isotype switching to IgA isotypes
Research Articles on TNFSF13
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Product Notes
The Mouse TNFSF13 tnfsf13 (Catalog #AAA9716726) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716726 ELISA Kit recognizes Mouse TNFSF13. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily Member 13 (TNFSF13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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