Mouse Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ELISA Kit | TNFSF13 elisa kit

Mouse Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ELISA Kit

Cat. Num. AAA454327

• Gene Names: Tnfsf13; April; Tall2; Trdl1; Tnlg7b; 2310026N09Rik
• Reactivity: Mouse
• Synonyms: Tumor Necrosis Factor Ligand Superfamily; Member 13 (TNFSF13); Mouse Tumor Necrosis Factor Ligand Superfamily; Member 13 (TNFSF13) ELISA Kit; CD256; APRIL; TALL2; TRDL1; ZTNF2; A Proliferation Inducing Ligand; TNF And APOL Related Leukocyte Expressed Ligand 2; TNF-Related Death Ligand 1; TNFSF13 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNFSF13. No significant cross-reactivity or interference between TNFSF13 and analogues was observed.
• Sequence Length: 240

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 46.87-3000pg/mL
• Sensitivity: 22.7pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFSF13 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFSF13 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF13 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFSF13 in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFSF13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNFSF13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226958398
• NCBI GeneID: 69583
• NCBI Accession #: NP_001152977.1
• NCBI GenBank Nucleotide #: NM_001159505.1
• UniProt Accession #: Q9D777; Q9ERP1
• Molecular Weight: 26,889 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13
• NCBI Official Symbol: Tnfsf13
• NCBI Official Synonym Symbols: April; Tall2; Trdl1; Tnlg7b; 2310026N09Rik
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf13
• UniProt Synonym Gene Names: April; APRIL

Uniprot Description

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.