Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protease serine 4 (TMPRSS4) Standard Curve.)
Mouse Transmembrane Protease Serine 4 (TMPRSS4) ELISA Kit | TMPRSS4 elisa kit
Mouse Transmembrane Protease Serine 4 (TMPRSS4) ELISA Kit
Gene Names
Tmprss4; CAP2; mCAP2
Reactivity
Mouse
Synonyms
Transmembrane Protease Serine 4 (TMPRSS4); Mouse Transmembrane Protease Serine 4 (TMPRSS4) ELISA Kit; TMPRSS4; MT-SP2; TMPRSS3; membrane-type serine protease 2; transmembrane serine protease 3; type membrane serine protease; TMPRSS4 elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane protease serine 4 (TMPRSS4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMPRSS4. No significant cross-reactivity or interference between Mouse TMPRSS4 and analogues was observed.
Sequence Length
435
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protease serine 4 (TMPRSS4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protease serine 4 (TMPRSS4) Standard Curve.)
Related Product Information for TMPRSS4 elisa kit
Description: This Mouse Transmembrane protease serine 4 (TMPRSS4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS4 in samples. An antibody specific for TMPRSS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMPRSS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMPRSS4 encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene.A weak TMPRSS4 signal was detected in normal tissues of the gastrointestinal tract and in some of the urogenital tract but was not expressed in any other normal tissues. The authors suggested that TMPRSS4 may be important in processes involved in metastasis formation and tumor invasion.
Background: TMPRSS4 encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene.A weak TMPRSS4 signal was detected in normal tissues of the gastrointestinal tract and in some of the urogenital tract but was not expressed in any other normal tissues. The authors suggested that TMPRSS4 may be important in processes involved in metastasis formation and tumor invasion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane protease serine 4 isoform 1
NCBI Official Synonym Full Names
transmembrane protease, serine 4
NCBI Official Symbol
Tmprss4
NCBI Official Synonym Symbols
CAP2; mCAP2
NCBI Protein Information
transmembrane protease serine 4
UniProt Protein Name
Transmembrane protease serine 4
Protein Family
UniProt Gene Name
Tmprss4
UniProt Synonym Gene Names
Cap2; mCAP2
UniProt Entry Name
TMPS4_MOUSE
Uniprot Description
TMPRSS4: Probable protease. Seems to be capable of activating ENaC. Belongs to the peptidase S1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.-; Membrane protein, integral; Protease
Cellular Component: membrane; integral to membrane
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity; scavenger receptor activity
Biological Process: proteolysis
Research Articles on TMPRSS4
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Product Notes
The Mouse TMPRSS4 tmprss4 (Catalog #AAA9716759) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716759 ELISA Kit recognizes Mouse TMPRSS4. It is sometimes possible for the material contained within the vial of "Transmembrane Protease Serine 4 (TMPRSS4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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