Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transketolase (TKT) Standard Curve.)
Mouse Transketolase (TKT) ELISA Kit | TKT elisa kit
Mouse Transketolase (TKT) ELISA Kit
Gene Names
Tkt; p68
Reactivity
Mouse
Synonyms
Transketolase (TKT); Mouse Transketolase (TKT) ELISA Kit; TKT; FLJ34765; TKT1; TKT elisa kit
Reactivity
Mouse
Specificity
Mouse Transketolase (TKT) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TKT. No significant cross-reactivity or interference between Mouse TKT and analogues was observed.
Sequence Length
623
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transketolase (TKT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transketolase (TKT) Standard Curve.)
Related Product Information for TKT elisa kit
Description: This Mouse Transketolase (TKT) ELISA Kit employs a two-site sandwich ELISA to quantitate TKT in samples. An antibody specific for TKT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TKT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TKT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TKT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Transketolase (EC 2.2.1.1) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.
Background: Transketolase (EC 2.2.1.1) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transketolase
NCBI Official Synonym Full Names
transketolase
NCBI Official Symbol
Tkt
NCBI Official Synonym Symbols
p68
NCBI Protein Information
transketolase
UniProt Protein Name
Transketolase
Protein Family
UniProt Gene Name
Tkt
UniProt Synonym Gene Names
TK
UniProt Entry Name
TKT_MOUSE
NCBI Description
This gene encodes an enzyme that binds magnesium and thiamine pyrophosphate and catalyzes the transfer of sugar phosphates to an aldose acceptor. This enzyme is a key component of the pentose phosphate pathway during glycolysis. It is significantly expressed in the cornea and may be involved in the cellular response against oxidative stress. Haploinsufficiency of this gene leads to decreased growth and reduction of adipose tissue. [provided by RefSeq, Dec 2013]
Research Articles on TKT
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Product Notes
The Mouse TKT tkt (Catalog #AAA9716826) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716826 ELISA Kit recognizes Mouse TKT. It is sometimes possible for the material contained within the vial of "Transketolase (TKT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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