Typical Testing Data/Standard Curve (for reference only)
Mouse klotho beta ELISA Kit | KLB elisa kit
Mouse Beta-klotho, KLB ELISA Kit
Gene Names
Klb; AV071179; betaKlotho
Reactivity
Mouse
Synonyms
klotho beta; Mouse Beta-klotho; KLB ELISA Kit; Mouse Beta-klotho (KLB) ELISA kit; BKL; MGC142213; klotho beta like; KLB elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse KLB. No significant cross-reactivity or interference between mouse KLB and analogues was observed.
Sequence Length
1,043
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of mouse KLB is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KLB elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLB present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for KLB is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,189 Da
NCBI Official Full Name
beta-klotho
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
Klb
NCBI Official Synonym Symbols
AV071179; betaKlotho
NCBI Protein Information
beta-klotho; BKL; klotho beta-like protein
UniProt Protein Name
Beta-klotho
Protein Family
UniProt Gene Name
Klb
UniProt Synonym Gene Names
Betakl; BKL; BetaKlotho
UniProt Entry Name
KLOTB_MOUSE
Uniprot Description
Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Protein type: Membrane protein, integral
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: fibroblast growth factor binding; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding
Biological Process: fibroblast growth factor receptor signaling pathway; carbohydrate metabolic process; positive regulation of cell proliferation
Research Articles on KLB
Similar Products
Product Notes
The Mouse KLB klb (Catalog #AAA929245) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA929245 ELISA Kit recognizes Mouse KLB. It is sometimes possible for the material contained within the vial of "klotho beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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