Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant PIIINP, Mouse)
Rabbit Procollagen III N-Terminal Propeptide (PIIINP) Polyclonal Antibody | anti-PIIINP antibody
Polyclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant PIIINP, Mouse)
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant PIIINP, Mouse)
Immunohistochemistry-Paraffin (IHC-P)
(DAB staining on IHC-P; Sample: Mouse Uterus Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (MBS2086047))
Immunohistochemistry-Paraffin (IHC-P)
(DAB staining on IHC-P; Sample: Mouse Uterus Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (MBS2086047))
Immunohistochemistry-Paraffin (IHC-P)
(DAB staining on IHC-P; Sample: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (MBS2086047))
Immunohistochemistry-Paraffin (IHC-P)
(DAB staining on IHC-P; Sample: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015]
Uniprot Description
CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1 C1.1|1 23.67 cM
Cellular Component: collagen; collagen type III trimer; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: extracellular matrix structural constituent; integrin binding; metal ion binding; platelet-derived growth factor binding; protease binding; SMAD binding
Biological Process: blood vessel development; cell-matrix adhesion; cerebral cortex development; collagen fibril organization; gut development; heart development; integrin-mediated signaling pathway; negative regulation of immune response; negative regulation of neuron migration; peptide cross-linking; positive regulation of Rho protein signal transduction; response to cytokine; response to radiation; skin development; transforming growth factor beta receptor signaling pathway; wound healing
Research Articles on PIIINP
Similar Products
Product Notes
The PIIINP col3a1 (Catalog #AAA2090489) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Procollagen III N-Terminal Propeptide (PIIINP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blotting: 0.5-2 ug/mL Immunohistochemistry: 5-20 ug/mL Immunocytochemistry: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PIIINP col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Procollagen III N-Terminal Propeptide (PIIINP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
