Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF25/DR3 PicoKine ELISA Kit standard curve )
Mouse TNFRSF25 ELISA Kit | TNFRSF25 elisa kit
Mouse TNFRSF25/DR3 PicoKine ELISA Kit
Gene Names
Tnfrsf25; DR3; TR3; Wsl; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; Tnfrsf12
Reactivity
Mouse
Synonyms
TNFRSF25; Mouse TNFRSF25/DR3 PicoKine ELISA Kit; Apo3; Apo-3; Apo 3; DDR3; DDR 3; DR 3; LARD; TNFRSF 12; TNFRSF12; TNFRSF 25; TR 3; TR3; TRAMP; WSL 1; WSL1; WSLLR; WSL LR; B1AWN9; Protein Tnfrsf25; tumor necrosis factor receptor superfamily member 25; TNFRSF25 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse TNFRSF25/DR3
Sequence Length
411
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF25/DR3 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF25/DR3 PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF25 elisa kit
Principle of the Assay: Mouse TNFRSF25/DR3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF25/DR3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q31-F199) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF25/DR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF25/DR3 amount of sample captured in plate.
Background/Introduction: Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
Background/Introduction: Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,264 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 25 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 25
NCBI Official Symbol
Tnfrsf25
NCBI Official Synonym Symbols
DR3; TR3; Wsl; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; Tnfrsf12
NCBI Protein Information
tumor necrosis factor receptor superfamily member 25
UniProt Protein Name
Protein Tnfrsf25
Protein Family
UniProt Gene Name
Tnfrsf25
UniProt Entry Name
B1AWN9_MOUSE
Research Articles on TNFRSF25
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Product Notes
The Mouse TNFRSF25 tnfrsf25 (Catalog #AAA178554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178554 ELISA Kit recognizes Mouse TNFRSF25. It is sometimes possible for the material contained within the vial of "TNFRSF25, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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