Human TNFRSF25/DR3 ELISA Kit | TNFRSF25 elisa kit

Human TNFRSF25/DR3 PicoKine ELISA Kit

Cat. Num. AAA178765

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human
• Synonyms: TNFRSF25/DR3; Human TNFRSF25/DR3 PicoKine ELISA Kit; Apo3; Apo-3; Apo 3; DDR3; DDR 3; DR 3; LARD; TNFRSF 12; TNFRSF12; TNFRSF 25; TNFRSF25; TR 3; TR3; TRAMP; WSL 1; WSL1; WSLLR; WSL LR; Q93038; Tumor necrosis factor receptor superfamily member 25; TNF receptor superfamily member 25; TNFRSF25 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TNFRSF12A
• Sequence Length: 181

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFRSF25 elisa kit

Principle of the Assay: The Picokine Human TNFRSF12A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFRSF12A with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF12A. The detection antibody is a biotinylated antibody specific for TNFRSF12A. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human TNFRSF12A with immunogen: Expression system for standard: E.coli, Immunogen sequence: E28-P80. The kit is analytically validated with ready to use reagents. To measure Human TNFRSF12A, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TNFRSF12A in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF12A in the sample!!Background/Introduction: The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 89142745
• NCBI GeneID: 8718
• NCBI Accession #: NP_001034753.1
• NCBI GenBank Nucleotide #: NM_001039664.1
• UniProt Accession #: Q93038; O00275; O00276; O00277; O00278; O00279; O00280; O14865; O14866; B1ALX2; B1ALX3; B7ZLL7
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 12
• NCBI Official Synonym Full Names: TNF receptor superfamily member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.