Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tissue inhibitors of metalloproteinase 1 (TIMP1) Standard Curve.)
Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit | TIMP1 elisa kit
Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit
Gene Names
Timp1; EPA; Clgi; Timp; TIMP-1; TPA-S1
Reactivity
Mouse
Synonyms
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1); Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit; TIMP1; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; Metalloproteinase inhibitor 1; TIMP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIMP1. No significant cross-reactivity or interference between Mouse TIMP1 and analogues was observed.
Sequence Length
205
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tissue inhibitors of metalloproteinase 1 (TIMP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tissue inhibitors of metalloproteinase 1 (TIMP1) Standard Curve.)
Related Product Information for TIMP1 elisa kit
Description: This Mouse Tissue inhibitors of metalloproteinase 1 (TIMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP1 in samples. An antibody specific for TIMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIMP metallopeptidase inhibitor 1, a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Background: TIMP metallopeptidase inhibitor 1, a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
metalloproteinase inhibitor 1 isoform a
NCBI Official Synonym Full Names
tissue inhibitor of metalloproteinase 1
NCBI Official Symbol
Timp1
NCBI Official Synonym Symbols
EPA; Clgi; Timp; TIMP-1; TPA-S1
NCBI Protein Information
metalloproteinase inhibitor 1
UniProt Protein Name
Metalloproteinase inhibitor 1
Protein Family
UniProt Gene Name
Timp1
UniProt Synonym Gene Names
Timp; Timp-1; EPA; TIMP-1
UniProt Entry Name
TIMP1_MOUSE
Uniprot Description
TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: basement membrane; extracellular matrix; extracellular space; proteinaceous extracellular matrix
Molecular Function: cytokine activity; metalloendopeptidase inhibitor activity; protease binding
Biological Process: cell activation; negative regulation of apoptosis; negative regulation of catalytic activity; negative regulation of membrane protein ectodomain proteolysis; positive regulation of cell proliferation; response to cytokine stimulus; response to hormone stimulus
Research Articles on TIMP1
Similar Products
Product Notes
The Mouse TIMP1 timp1 (Catalog #AAA9716836) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716836 ELISA Kit recognizes Mouse TIMP1. It is sometimes possible for the material contained within the vial of "Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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