Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyrotropin-releasing hormone (TRH) Standard Curve.)
Mouse Thyrotropin-Releasing Hormone (TRH) ELISA Kit | TRH elisa kit
Mouse Thyrotropin-Releasing Hormone (TRH) ELISA Kit
Gene Names
Trh; Trf; Pro-TRH
Reactivity
Mouse
Synonyms
Thyrotropin-Releasing Hormone (TRH); Mouse Thyrotropin-Releasing Hormone (TRH) ELISA Kit; TRH; MGC125964; MGC125965; TRH elisa kit
Reactivity
Mouse
Specificity
Mouse Thyrotropin-releasing hormone (TRH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRH. No significant cross-reactivity or interference between Mouse TRH and analogues was observed.
Sequence Length
256
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyrotropin-releasing hormone (TRH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyrotropin-releasing hormone (TRH) Standard Curve.)
Related Product Information for TRH elisa kit
Description: This Mouse Thyrotropin-releasing hormone (TRH) ELISA Kit employs a two-site sandwich ELISA to quantitate TRH in samples. An antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thyrotropin releasing hormone, a hormone secreted by the hypothalamus that stimulates release of thyrotropin. TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets. Thyroid-stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs. The most important controller of TSH secretion is thyroid-releasing hormone. TRH is produced by the hypothalamus in medial neurons of the paraventricular nucleus. Initially, it is synthesized as a 242 amino acid precursor polypeptide that contains 6 copies of the sequence -Gln-His-Pro-Gly-, flanked by di-basic peptides that are later processed through proteolysis to give the mature TRH molecule.
Background: Thyrotropin releasing hormone, a hormone secreted by the hypothalamus that stimulates release of thyrotropin. TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets. Thyroid-stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs. The most important controller of TSH secretion is thyroid-releasing hormone. TRH is produced by the hypothalamus in medial neurons of the paraventricular nucleus. Initially, it is synthesized as a 242 amino acid precursor polypeptide that contains 6 copies of the sequence -Gln-His-Pro-Gly-, flanked by di-basic peptides that are later processed through proteolysis to give the mature TRH molecule.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin releasing hormone
NCBI Official Symbol
Trh
NCBI Official Synonym Symbols
Trf; Pro-TRH
NCBI Protein Information
thyrotropin releasing hormone
UniProt Protein Name
Pro-thyrotropin-releasing hormone
Protein Family
UniProt Gene Name
Trh
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF
NCBI Description
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013]
Uniprot Description
Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
Research Articles on TRH
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Product Notes
The Mouse TRH trh (Catalog #AAA9716668) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716668 ELISA Kit recognizes Mouse TRH. It is sometimes possible for the material contained within the vial of "Thyrotropin-Releasing Hormone (TRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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