Mouse Thrombopoietin (TPO) ELISA Kit | TPO elisa kit

Mouse Thrombopoietin (TPO) ELISA Kit

Cat. Num. AAA2019481

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Reactivity: Mouse
• Synonyms: Thrombopoietin (TPO); Mouse Thrombopoietin (TPO) ELISA Kit; THPO; MGDF; MKCSF; ML; MPLLG; Myeloproliferative Leukemia Virus Oncogene Ligand; Megakaryocyte Growth And Development Factor; Megakaryocyte Stimulating Factor; TPO elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thrombopoietin (TPO).; No significant cross-reactivity or interference between Thrombopoietin (TPO) and analogues was observed.
• Sequence Length: 353

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 3.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombopoietin (TPO) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombopoietin (TPO) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombopoietin (TPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombopoietin (TPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombopoietin (TPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombopoietin (TPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TPO elisa kit

Hematology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 914226
• NCBI GeneID: 7066
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
• Molecular Weight: 33,604 Da
• NCBI Official Full Name: thrombopoietin
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: platelet activation; cell proliferation; positive regulation of protein kinase B signaling cascade; multicellular organismal development; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1