Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sulfhydryl oxidase 1 (QSOX1) Standard Curve.)
Mouse Sulfhydryl Oxidase 1 (QSOX1) ELISA Kit | QSOX1 elisa kit
Mouse Sulfhydryl Oxidase 1 (QSOX1) ELISA Kit
Gene Names
Qsox1; SOx; QSOX; Qscn6; b2b2673Clo; 1300003H02Rik
Reactivity
Mouse
Synonyms
Sulfhydryl Oxidase 1 (QSOX1); Mouse Sulfhydryl Oxidase 1 (QSOX1) ELISA Kit; QSOX1; FLJ34858; Q6; QSCN6; QSOX1 elisa kit
Reactivity
Mouse
Specificity
Mouse Sulfhydryl oxidase 1 (QSOX1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse QSOX1. No significant cross-reactivity or interference between Mouse QSOX1 and analogues was observed.
Sequence Length
748
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sulfhydryl oxidase 1 (QSOX1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sulfhydryl oxidase 1 (QSOX1) Standard Curve.)
Related Product Information for QSOX1 elisa kit
Description: This Mouse Sulfhydryl oxidase 1 (QSOX1) ELISA Kit employs a two-site sandwich ELISA to quantitate QSOX1 in samples. An antibody specific for QSOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QSOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QSOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sulfhydryl oxidase 1 is a protein that contains domains of thioredoxin and ERV1, members of two long-standing gene families. The gene expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation. Two transcript variants encoding two different isoforms have been found for this gene. Coppock et al. (1993) isolated human embryo lung fibroblast cDNAs that were expressed at a higher level in quiescent cells than in logarithmically growing cells. Several partial cDNAs corresponded to the QSOX1 gene, which the authors called Q6. Northern blot analysis revealed that Q6 is expressed as 3- and 4-kb mRNAs in embryo lung fibroblasts.
Background: Sulfhydryl oxidase 1 is a protein that contains domains of thioredoxin and ERV1, members of two long-standing gene families. The gene expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation. Two transcript variants encoding two different isoforms have been found for this gene. Coppock et al. (1993) isolated human embryo lung fibroblast cDNAs that were expressed at a higher level in quiescent cells than in logarithmically growing cells. Several partial cDNAs corresponded to the QSOX1 gene, which the authors called Q6. Northern blot analysis revealed that Q6 is expressed as 3- and 4-kb mRNAs in embryo lung fibroblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sulfhydryl oxidase 1 isoform a
NCBI Official Synonym Full Names
quiescin Q6 sulfhydryl oxidase 1
NCBI Official Symbol
Qsox1
NCBI Official Synonym Symbols
SOx; QSOX; Qscn6; b2b2673Clo; 1300003H02Rik
NCBI Protein Information
sulfhydryl oxidase 1
UniProt Protein Name
Sulfhydryl oxidase 1
Protein Family
UniProt Gene Name
Qsox1
UniProt Synonym Gene Names
Qscn6; Sox; mSOx
Uniprot Description
Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins ().
Research Articles on QSOX1
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Product Notes
The Mouse QSOX1 qsox1 (Catalog #AAA9717187) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717187 ELISA Kit recognizes Mouse QSOX1. It is sometimes possible for the material contained within the vial of "Sulfhydryl Oxidase 1 (QSOX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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