Fibroblast Growth Factor 19 (FGF19) CLIA Kit | FGF19 clia kit

CLIA Kit for Fibroblast Growth Factor 19 (FGF19)

Cat. Num. AAA2000692

• Synonyms: Fibroblast Growth Factor 19 (FGF19); CLIA Kit for Fibroblast Growth Factor 19 (FGF19); FGF19 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 19 (FGF19); No significant cross-reactivity or interference between Fibroblast Growth Factor 19 (FGF19) and analogues was observed.
• Sequence Length: 216

• Assay Type: Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 1.372-1000pg/mL The standard curve concentrations used for the ELISA's were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.346pg/mL, 4.115pg/mL, 1.372pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 19 (FGF19) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 19 (FGF19) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Rattus norvegicus (Rat)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF19 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 19 (FGF19) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 4826726
• NCBI GeneID: 9965
• NCBI Accession #: NP_005108.1
• NCBI GenBank Nucleotide #: NM_005117.2
• UniProt Accession #: O95750
• Molecular Weight: 24,003 Da
• NCBI Official Full Name: fibroblast growth factor 19
• NCBI Official Synonym Full Names: fibroblast growth factor 19
• NCBI Official Symbol: FGF19
• NCBI Protein Information: fibroblast growth factor 19; FGF-19
• UniProt Protein Name: Fibroblast growth factor 19
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF19
• UniProt Synonym Gene Names: FGF-19
• UniProt Entry Name: FGF19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; positive regulation of glucose import; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; neural crest cell migration