Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Blood Cells; Lane2: Porcine Blood Cells.)
Rabbit anti-Human Activating Transcription Factor 1 Polyclonal Antibody | anti-ATF1 antibody
Polyclonal Antibody to Activating Transcription Factor 1 (ATF1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV TLTSQTTKTD DPQ
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Blood Cells; Lane2: Porcine Blood Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Blood Cells; Lane2: Porcine Blood Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ATF1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ATF1, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
Uniprot Description
ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: ATF1-ATF4 transcription factor complex; nucleoplasm; nucleus
Molecular Function: DNA binding transcription factor activity; identical protein binding; protein binding; protein complex binding; protein heterodimerization activity; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
Biological Process: cellular protein complex assembly; positive regulation of DNA replication; positive regulation of neuron projection development; positive regulation of transcription from RNA polymerase II promoter; response to cobalt ion; response to organic cyclic compound; transcription from RNA polymerase II promoter
Research Articles on ATF1
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Product Notes
The ATF1 atf1 (Catalog #AAA2028444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Activating Transcription Factor 1 (ATF1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Activating Transcription Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ATF1 atf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV TLTSQTTKTD DPQ. It is sometimes possible for the material contained within the vial of "Activating Transcription Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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