Western Blot (WB)
(Western Blot Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: PTPN11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 53 kDa Observed band size: 69 kDa)
PTPN11 recombinant antibody
PTPN11 Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: PTPN11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 53 kDa Observed band size: 69 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: PTPN11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 53 kDa Observed band size: 69 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Uniprot Description
SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.
Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: protein complex; mitochondrion; cytoplasm; cytosol; nucleus
Molecular Function: protein domain specific binding; protein binding; phospholipase binding; SH3/SH2 adaptor activity; peptide hormone receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; phosphoprotein phosphatase activity; D1 dopamine receptor binding; insulin receptor binding
Biological Process: axon guidance; nerve growth factor receptor signaling pathway; hormone-mediated signaling; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; reproductive process in a multicellular organism; glucose homeostasis; regulation of protein export from nucleus; negative regulation of insulin secretion; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; inner ear development; negative regulation of cell adhesion mediated by integrin; negative regulation of growth hormone secretion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; brain development; platelet formation; blood coagulation; leukocyte migration
Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1
Research Articles on PTPN11
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Product Notes
The PTPN11 ptpn11 (Catalog #AAA7136634) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPN11 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PTPN11 ptpn11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN11, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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