Mouse SOST (Sclerostin) ELISA Kit | SOST elisa kit

Mouse SOST (Sclerostin) ELISA Kit

Cat. Num. AAA7607262

• Gene Names: SOST; CDD; VBCH; DAND6; SOST1
• Reactivity: Mouse
• Synonyms: SOST (Sclerostin); Mouse SOST (Sclerostin) ELISA Kit; SOST elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOST. No significant cross-reactivity or interference between SOST and analogues was observed.
• Sequence Length: 213

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: <37.5pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOST were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOST were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOST elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SOST antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SOST antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SOST amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SOST can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13376846
• NCBI GeneID: 50964
• NCBI Accession #: NP_079513.1
• NCBI GenBank Nucleotide #: NM_025237.3
• UniProt Accession #: Q9BQB4; Q495N9
• Molecular Weight: 24,264 Da
• NCBI Official Full Name: sclerostin
• NCBI Official Synonym Full Names: sclerostin
• NCBI Official Symbol: SOST
• NCBI Official Synonym Symbols: CDD; VBCH; DAND6; SOST1
• NCBI Protein Information: sclerostin
• UniProt Protein Name: Sclerostin
• Protein Family: Sclerostin
• UniProt Gene Name: SOST

NCBI Description

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.